Rapid SDS-PAGE Gel kit

Catalog number:E-IR-R306

Introduction:
Elabscience® Rapid SDS-PAGE Gel Kit is a low acrylamide product which uses the latest gel technology to reduce acrylamide concentration and improve gel speed.
The kit is simple to operate with simple in gel preparation, fast and safe in rubber distribution. Separating gel and stacking gel are prepared at the same time which makes the whole process of gel preparation completed in 25 min.
The produced SDS-PAGE gel can be rapidly electrophoretic at high voltage, with 300V Voltage the whole electrophoresis time can be shortened in 25 min. It also can carry out conventional voltage electrophoresis.
This kit is suitable to isolate 10-250 kDa protein without adjusting the separating gel concentration according to the molecular weight of the proteins.

Experimental Procedure
1.Mix the Separating Gel Mix A [E-IR-R306C] and Separating Gel Mix B [E-IR-R306D] in a ratio of 1:1 (Reference: 0.75/1.0/1.5 mm for each piece of gel, 2.0/2.5/3.8 mL of Separating Gel Mix A and Separating Gel Mix are needed respectively).
2.Mix the Stacking Gel Mix A [E-IR-R306A] and Stacking Gel Mix B [E-IR-R306B] in a ratio of 1:1 (Reference: 0.75/1.0/1.5 mm for each piece of gel, 0.8/1.0/1.5 mL of Stacking Gel Mix A and Separating Gel Mix are needed respectively).
TIP: This product is rapid gel. Please configure the gel components according to the instruction manual. Do not mix the Stacking gel after pouring the Separating Gel into the model. Too long time waiting will lead to partial polymerization of the separating gel, resulting in the uneven line between the separating and stacking gel which will affect the electrophoresis effect.
3.Add 10% APS Buffer to the Separating Gel Mixture produced in step 1 according to the ratio of 1:100 (e.g. add 50 μL 10% APS Buffer to 5 mL separating gel mixture), mix well and then pour it into the model.
TIP: If the room temperature is high or it is necessary to prepare multi gels at one time. The amount of APS can be reduced properly (for example, adding 10% APS of 40 L per 5 mL separating gel mixture) to reduce gel speed. Excessive APS causes separating gel to become brittle.
4.Add 10% APS Buffer to the Stacking Gel Mixture produced in step 2 according to the ratio of 1:100 (e.g. add 20 μL 10% APS Buffer to each 2 mL separating gel mixture), mix well and then pour it into the model above the Separating Gel Mixture.
TIP: This kit can complete gel preparation at one time without mutual mixing of Stacking and separating gel.
5.Insert comb carefully and avoid bubbles, wait for 15~20 min till the gel polymerizes completely.
6.Take out the comb and add the samples of protein to each well. It is recommended that Elabscience® Pre-stained Protein Marker (10-180 kDa) [E-BC-R273] can be used during the experiment to indicated Molecular weight on the PAGE gel.
7.The product can be used for rapid electrophoresis in conventional electrophoretic buffer. The maximum voltage can be set to 300 V and the electrophoresis can be completed in about 25 minutes. The maximum current should not exceed 140 mA. If the current is too large please reduce the voltage.
TIP: It also can carry out conventional voltage electrophoresis.

Storage
Store at 2~8°C in dark. Avoid of freezing. Valid for 12 months.
Add 5 mL ddH2O to the APS powder [E-IR-R306F] to prepare the 10% APS Buffer before the experiment. Split charging and store at -20°C.

Cautions
1.For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
2.In the process of preparation, bubbles should be avoided to avoid affecting the experimental results.
3.This kit is for research use only. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
4.This kit is rapid SDS-PAGE gel kit. Be sure to follow the Experimental Procedure according to this manual. Add the Stacking Gel Mixture immediately after the Separating Gel Mixture added into the model to avoid the Separating Gel Mixture polymerizing which may cause uneven line between the separating and stacking gel.
5.This kit is suitable to isolate 10-250 kDa protein without adjusting the separating gel concentration according to the size of the protein.