PBS Buffer, pH7.4 (10 ×)

Catalog number:E-BC-R187

Introduction
PBS buffer is a phosphate buffer, which provides a relatively stable ionic environment and pH buffering capacity and is the most commonly used buffer for biochemistry. When you prepare the WB samples, pre-cooled PBS are used to wash the surface of tissues and internal debris or cell culture medium to prevent the interference of endogenous IgG or culture medium.

Instructions
This product is a 10 × concentrated solution, dilute with pure water to 1 × working solution before use.
For example: take 100 mL PBS buffer (10 × ), dilute with pure water to 1 L.

1 × PBS buffer component
136.9 mM NaCl, 2.68 mM KCl, 10 mM Na2HPO4•12H2O, 2 mM KH2PO4, pH 7.4.

Storage
Store at room temperature for 12 months.

Cautions
1. PBS may precipitate when stored at low temperature. If PBS precipitates, please dissolve PBS in water bath at 37°C, and then dilute it to 1 x working buffer.
2. Keep the product sealed to prevent from pollution.
3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.