NGS LIBRARY QUANTIFICATION KIT

Simple to use kit for checking your NGS library quantity and quality before you sequence it.

The YouSeq NGS Library quantification kit employs qPCR as a highly specific and Sensitive method to calculate the amount of DNA library present with successfully incorporated adapters. This kit will help improve library loading concentration and therefore, improve sequencing result for each patient so reducing risk of patient repeats.
qPCR is the only technically capable of a library quantification of total sequencable product. Other technologies will include non-specific products in the quantification meaning reduction in quantification accuracy.

Results are compared to a set of calibrated standards for precise quantification.

Dilution buffer is also supplied to dilute your DNA library accordingly, ready to be sequenced.

Kit contents:
- Library specific qPCR primers
- qPCR MasterMix premixed with SYBRgreen
- 4 x positive control templates to create standard curve
- NGS library dilution buffer

Application
Specifically quantifies sequence ready library only:
- Precise across a very broad dynamic range
- Ideal for automated liquid handling
- Less expensive than alternative methods
- Highly sensitive- accurate quantification of very dilute libraries
- Consumes only small amounts of sample

Technical specification:
qPCR is the most accurate method for quantification of an NGS Library. It is also far more cost effective and requires less bench time, especially in higher throughput laboratories vs Bioanalyzer of Spectrophotometer.