TransTaq DNA Polymerase High Fidelity contains TransTaq-T DNA Polymerase and a proofreading 3′-5′ exonuclease. Two different buffers are provided in the kit. TransTaq HiFi Buffer I is optimez for the amplification of genomic DNA and TransTaq HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
i-StarTaq ™ DNA Polymerase is a non-antibody, chemical-based, hot-start enzyme that reduces nonspecific amplification and improves specificity and accuracy. Therefore, it can be used as a solution when the specific band is weakly amplified by nonspecific amplification such as nonspecific band or primer dimer.Although Taq DNA Polymerase is most commonly used in PCR, as the number of targets to be amplified varies, Taq DNA Ppolymerase is not suitable for some PCR conditions. The most frequently encountered problem in PCR is that the non-specific band is amplified together or the amplification of the specific band is remarkably low or fails, mainly due to the low specificity. This happens because either the forward or reverse primer is less specific or the two primers are more prone to primer dimers because Taq DNA Polymerase is usually active at room temperature to prepare the PCR reaction solution.
2x PCR Master Mix Solution (i-MAXTM II) is a product designed to achieve the best results with simple 2x master mix type in one tube, including all components necessary for PCR reaction including i-MAXTM II DNA Polymerase is. This product is able to perform PCR by adding template DNA, primer set and D.W only, and gel loading buffer is included, so gel loading can be done without any other treatment. The i-MAXTM II DNA Polymerase used in this product has the advantages of Taq DNA Polymerase and Pfu DNA Polymerase to overcome the disadvantages of each enzyme and to obtain new functions. It has a proofreading function, Kb) to 20 Kb or more.
Dried & aliquoted PreMix Kit of i-StarTaq™ GH DNA Polymerase with high specificity and high amplification efficiency with Antibody based-Hot Start method Dried & aquoted PreMix, All-in-one products. All the reagents required for the reaction are in dried pellet in one tube. If template, primer and D.W are added, PCR will be performed immediately. Gel loading electrophoresis is possible because it contains loading dye. Optimal buffering system for high amplification efficiency High-purity Hot-Start Taq DNA Polymerase Suitable for a wide range of applications requiring high amplification efficiency and specificity Maxime PCR PreMix Kit (i-StarTaq™ GH) is a product with excellent specificity, sensitivity and amplification rate using i-StarTaq™ GH DNA Polymerase. The i-StarTaq™ GH DNA Polymerase used in this product inhibits the enzyme activity at low temperature using the antibody-based Hot Start method.
ONE-STEP RT-PCR PreMix Kit is a master mix type ONE-STEP RT-PCR PreMix Kit that can perform cDNA synthesis and PCR reaction all at once using gene specific primers. The general RT-PCR method is a two-step sequential reaction, cDNA synthesis using reverse transcriptase, oligo dT / random primers and PCR process with DNA polymerase. The method is suitable for gene expression studies, but it has drawbacks such as cross contamination in complex processes. Gene specific primer (GSP) is preferred for the confirmation of the existence of specific genes and quantitative / qualitative analysis of the expressed genes. It is important to minimize errors in the reaction by simplifying the experimental procedure. The ONE-STEP RT-PCR PreMix Kit was designed to synthesize first-strand cDNA from total RNA or mRNA template and do PCR in a tube. The product includes all components, reverse transcriptase and DNA polymerase, for each reaction with mixed solution type. The OptiScriptTM RT System of ONE-STEP RT-PCR PreMix Kit makes accurate cDNA and high specificity PCR from 1pg to 2mg of template RNA. The ONE-STEP RT-PCR PreMix Kit is optimized product to confirm the expression of low copy gene, and its stability is maintained for the first time under frozen condition for more than 1 year.
RealMOD™ Green AP 5x qPCR mix is a 5x concentration premix type reagent, which contains all necessary components for Real-time PCR reaction except for primers and template DNA. Intercalating dye in the master mix enables the analysis of many different target genes. This product has the effect of suppressing primer-dimer formation, which is especially important matter in intercalating dye assay and this effect makes possible the accurate quantitative analysis in a wide range concentration of template DNA by minimizing non-specific amplification. RealMOD™ Green AP 5x qPCR mix is an optimized ready-to-use solution for real time quantitative PCR assays, including EvaGreen® dye, and the solution is activated by 12 minutes incubation at 95℃. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimer formation at low temperatures during qPCR setup.
RealMOD ™ Green W2 2x qPCR mix is a quantitative PCR product using cDNA or gDNA. This product has a hot start function, low dimer production rate, high specificity and high reproducibility. It is particularly sensitive and has very low signal-to-noise due to primer dimers. In addition, it is possible to experiment with template with high GC contents and it is effective with target of low copy number. This product is available in 2x type.
The RedSafe™ Nucleic Acid Staining Solution exhibits two fluorescence excitation maxima at 309 nm and 419 nm when bound to nucleic acids and is visible at a wavelength of 514 nm. RedSafe™ Nucleic Acid Staining Solution is as sensitive as EtBr and its usage is similar to EtBr. Compared with EtBr, a potent carcinogen, RedSafe™ Nucleic Acid Staining Solution showed almost no mutation in the Ames test and negative results in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Therefore, we strongly recommend that you select RedSafe™ Nucleic Acid Stainig Solution (20,000x) instead of EtBr to identify nucleic acids on agarose gel.
Sizer ™ -1000 plus DNA Marker Solution is able to confirm the size when nucleic acid electrophoresis is performed, and DNA in the range of 100 bp to 10,000 bp can be confirmed. A total of 15 fragments, namely 100/200/300/400/500/700 / 1,000 / 1,500 / 2,000 / 3,000 / 4,000 / It is composed of 5,000 / 6,000 / 8,000 / 10,000 bp. Each band has a concentration of 40 ng per 5 μl. A band of 500 bp and a band of 3,000 bp consist of a concentration of 100 ng, making it easy to distinguish the size. In addition, since the loading buffer is included with the marker, it can be used immediately without any additional formulation before use. It can be stained with its proprietary RedSafe ™ Nucleic Acid Staining Solution (Cat. No. 21141), ethidium bromide (EtBr) and other DNA stains.
The e-Myco™ VALiD Mycoplasma PCR Detection Kit is composed a set of primers those are specific for the highly conserved mycoplasma 16S-rRNA coding region including M. pneumoniae, M. agninini, M. hyorhinis, M. fermentans, M. orale and A. laidlawii. The kit is design to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Also, the kit can be performed in 3 hours, can detect sensitively until 10 CFU/ml and includes internal control for verifying a PCR run as well as positive control DNA. The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs (PCR product).
PRO-PREP™ Protein Extraction Solution can be simply extract protein from all kinds of cells and tissues. The kit contains 5 kinds of protease inhibitors so it is possible to extract very highly purified proteins. Usually detergent used in protein extraction consists of both hydrophobic tails and amphiphilic molecule and hydrophilic head. The two parts are joined to form a micelle. It is solubilized protein forming a lipid detergent mixed micelle and transmembrane protein forming a protein lipid detergent complex. The extent of micelle formation is termed as CMC(critical micelle concentration). Which is important for high efficiency as well as high purity of protein extraction. CMC is influenced by pH, temperature, ionic strength, multivalent ions of organic solvents, purity of detergent and so on. Depending on ionic characteristics, a detergent can be categorized as ionic detergent, non-ionic detergent, and Zwitterionic detergent
GangNam-STAIN™ Prestained Protein Ladder is a protein marker that can identify the protein size during protein electrophoresis with 12 bands between 10~245 kDa. It is specially efficient to use by two reference bands (Green:25 kDa, Red:75 kDa) and possible to use directly without pre-treatment such as buffer dilution. GangNam-Stain™ Prestained Protein Ladder is very suitable for protein separation from SDS-PAGE, confirmation of Western blot transfer efficiency, CBB staining and silver staining.
WEST-QueenTM Western Blot Detection System is a light emitting non-radioactive method for detection of immobilized specific antigen in chemiluminescent Western blots through horseradish peroxidase (HRP) labeled antibodies. Overlaying an X-ray film or visualization with a CCD camera is employed to detect luminescence corresponding to the protein band labeled by the antibodies.
easy-BLUE™ Total RNA Extraction Kit is a solution type that can extract total RNA easily and quickly from solid samples such as cell, tissue, and plant etc. In order to efficiently extract RNA from cells and tissues, it is necessary to effectively dissolve the cells or tissues and denature nucleic acid proteins, also inhibit the activity of RNase from cells. From this point of view, the easy-BLUE™ Total RNA Extraction Kit is the product that can most efficiently extract the RNA from tissues or cells. It is theoretically possible to extract pure RNA with almost no contamination of protein and genomic DNA when the amount of RNA extraction sample is appropriate.
The Patho Gene-spin™ DNA/RNA Extraction Kit uses a low concentration of chaotropic salt and has a better lysis efficiency unlike conventional Total RNA Extraction products. Total DNA/RNA can be extracted from various pathogen samples like virus and bacteria, even anticoagulated plasma/blood, serum, cell-free body fluids and pathogenic cell/tissue etc. And it is easy to induce lysis without any additive like mercaptoethanol. In addition, DNA/RNA extraction is faster and more efficient by using columns that silica-gel membrane technology applied. The DNA/RNA isolated by the Patho Gene-spin™ Kit can be used for clinical diagnosis of people and animals infected with diseases caused by viruses and bacteria.
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